Properties of Thiol-Subtilisin THE CONSEQUENCES OF CONVERTING THE ACTIVE SERINE RESIDUE TO CYSTEINE IN A SERINE PROTEASE

作者: K E Neet , A Nanci , D E Koshland

DOI: 10.1016/S0021-9258(18)93152-2

关键词: ChemistryProteolytic enzymesThiolSerine proteaseSubtilisinStereochemistryCysteineResidue (chemistry)SerineActive site

摘要: … whether the initial binding of substratt to enzyme is impaired in thiol-subtilisin, the dissociation constants of various compounds with subtilisin and thiolsuhtilisin have been measured. …

参考文章(38)
DW Ingles, JR Knowles, The α-chymotryptic hydrolysis of glycine esters Biochemical Journal. ,vol. 99, pp. 275- 282 ,(1966) , 10.1042/BJ0990275
Emil L. Smith, M.J. Parker, Kinetics of papain action. III. Hydrolysis of benzoyl-L-arginine ethyl ester. Journal of Biological Chemistry. ,vol. 233, pp. 1387- 1391 ,(1958) , 10.1016/S0021-9258(18)49346-5
William P. Jencks, Joan Carriuolo, Imidazole catalysis. III. General base catalysis and the reactions of acetyl imidazole with thiols and amines. Journal of Biological Chemistry. ,vol. 234, pp. 1280- 1285 ,(1959) , 10.1016/S0021-9258(18)98173-1
Hiroshi Matsubara, Charles B. Kasper, Douglas M. Brown, Emil L. Smith, Subtilisin BPN' Journal of Biological Chemistry. ,vol. 240, pp. 1125- 1130 ,(1965) , 10.1016/S0021-9258(18)97548-4
A.N. Glazer, Transesterification Reactions Catalyzed by Subtilisins Journal of Biological Chemistry. ,vol. 241, pp. 635- 638 ,(1966) , 10.1016/S0021-9258(18)96885-7
Milton. Winitz, Jesse P. Greenstein, Chemistry of the amino acids Wiley. ,(1961)
C.H.W. Hirs, The oxidation of ribonuclease with performic acid. Journal of Biological Chemistry. ,vol. 219, pp. 611- 621 ,(1956) , 10.1016/S0021-9258(18)65721-7
H. Weiner, C.W. Batt, D.E. Koshland, A Change in Specificity of Chymotrypsin Caused by Chemical Modification of Methionine Residues Journal of Biological Chemistry. ,vol. 241, pp. 2687- 2693 ,(1966) , 10.1016/S0021-9258(18)96594-4