UV-induced mutagenesis in Escherichia coli SOS response: a quantitative model.

摘要: Escherichia coli bacteria respond to DNA damage by a highly orchestrated series of events known as the SOS response, regulated transcription factors, protein–protein binding, and active protein degradation. We present dynamical model UV-induced incorporating mutagenesis error-prone polymerase, Pol V. In our model, depends on combination two key processes: counting replication forks long-term memory associated with accumulation UmuD′. Together, these provide tight regulation mutagenesis, resulting, we show, in “digital” turn-on turn-off Our provides compact view topology design network, pinpointing specific functional role each regulatory processes. particular, suggest that recently observed second peak activity promoters regulon (Friedman et al., 2005, PLoS Biology 3(7): e238) is result positive feedback from V RecA filaments.