DNA fingerprinting of Ascochyta rabiei with synthetic oligodeoxynucleotides

摘要: The ascomycete fungus Ascochyta rabiei, an important pathogen of the grain legume crop chickpea (Cicer arietinum L.) in Mediterranean region, has not been adequately characterized molecular terms. We therefore used DNA fingerprinting, with synthetic oligodeoxynucleotides complementary to simple repetitive sequences, pathotype different isolates fungus. Six single-spored A. rabiei were first categorized using a host differential set nine genotypes. Seedlings inoculated under controlled environmental conditions, and disease severity was recorded 9 days after inoculation. extracted from vitro-grown mycelia six purified fungal isolates, restricted EcoRI, HinfI, MboII TaqI, fingerprinted radiolabeled (GATA)4, (GTG)5, (CA)8, (TCC)5, respectively. High levels polymorphism detected optimal enzyme/probe combinations that allow one discriminate between isolates. potential fingerprinting sequences can thus be expanded identification races pathotypes. characterization geographic distribution genetic variability pathotypes will facilitate selection suitable cultivars grown specific regions.