Nitrogen removal from wastewater by anaerobic methane-driven denitrification in a lab-scale reactor: heterotrophic denitrifiers associated with denitrifying methanotrophs.

摘要: Nitrite-dependent anaerobic methane oxidation (n-damo) is a newly discovered bioprocess that reduces nitrite to dinitrogen with as electron donor, which has promising potential remove nitrogen from wastewater. In this work, lab-scale sequencing batch reactor (SBR) was operated for 609 days the sole external donor. SBR, in synthetic wastewater removed continuously; final volumetric removal rate 12.22±0.02 mg N L−1 day−1 and percentage of 98.5 ± 0.2 %. Microbial community analysis indicated denitrifying methanotrophs dominated (60–70 %) population sludge. Notably, activity testing microbial both suggested heterotrophic denitrifiers existed throughout operation period. After 609 days, denitrification 17 ± 2 % n-damo 83 ± 2 %. A possible mutualism may be developed between associated heterotrophs through cross-feed. Heterotrophs live on products excreted by provide growth factors are required methanotrophs.