Overview of protein expression in Pichia pastoris.
作者： David R. Higgins
DOI: 10.1002/0471140864.PS0507S02
关键词: Protein expression 、 Yeast 、 Plasmid 、 Molecular biology 、 Transformation (genetics) 、 Escherichia coli 、 Heterologous 、 Biology 、 Heterologous expression 、 Computational biology 、 Pichia pastoris
摘要: Pichia pastoris is a methylotrophic yeast and can be used as heterologous expression system. This microorganism easy to manipulate Escherichia coli, but has many of the advantages eukaryotic (e.g., protein processing, folding, post-translational modifications), it faster, easier, cheaper use than other systems, such baculovirus or mammalian tissue culture. It also generally yields higher levels. overview discusses important considerations for pastoris, including strains expression, plasmids, transformation by integration, modifications. Examples are given finally, legal issues regarding patent rights in described.
sci-hub.se 参考文章(40)
S B Ellis, P F Brust, P J Koutz, A F Waters, M M Harpold, T R Gingeras, Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Molecular and Cellular Biology. ,vol. 5, pp. 1111- 1121 ,(1985) , 10.1128/MCB.5.5.1111
J M Cregg, K J Barringer, A Y Hessler, K R Madden, Pichia pastoris as a host system for transformations. Molecular and Cellular Biology. ,vol. 5, pp. 3376- 3385 ,(1985) , 10.1128/MCB.5.12.3376
M.J. Hagenson, K.A. Holden, K.A. Parker, P.J. Wood, J.A. Cruze, M. Fuke, T.R. Hopkins, D.W. Stroman, Expression of streptokinase in Pichia pastoris yeast Enzyme and Microbial Technology. ,vol. 11, pp. 650- 656 ,(1989) , 10.1016/0141-0229(89)90003-3
J. J. Clare, F. B. Rayment, S. P. Ballantine, K. Sreekrishna, M. A. Romanos, High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Nature Biotechnology. ,vol. 9, pp. 455- 460 ,(1991) , 10.1038/NBT0591-455
Kiyotaka Shiba, Tracy Ripmaster, Noriko Suzuki, Ralph Nichols, Paul Plotz, Tetsuo Noda, Paul Schimmel, Human Alanyl-tRNA Synthetase: Conservation in Evolution of Catalytic Core and Microhelix Recognition Biochemistry. ,vol. 34, pp. 10340- 10349 ,(1995) , 10.1021/BI00033A004
M. E. Digan, S. V. Lair, R. A. Brierley, R. S. Siegel, M. E. Williams, S. B. Ellis, P. A. Kellaris, S. A. Provow, W. S. Craig, G. Veliçelebi, M. M. Harpold, G. P. Thill, Continuous Production of a Novel Lysozyme via Secretion from the Yeast, Pichia pastoris Nature Biotechnology. ,vol. 7, pp. 160- 164 ,(1989) , 10.1038/NBT0289-160
K. Sreekrishna, Lynn Nelles, Rica Potenz, John Cruze, Paul Mazzaferro, Wayne Fish, Motohiro Fuke, Kathy Holden, Doris Phelps, High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry. ,vol. 28, pp. 4117- 4125 ,(1989) , 10.1021/BI00435A074
Manuel Rodríguez, Roger Rubiera, Manuel Penichet, Raquel Montesinos, José Cremata, Viviana Falcón, Giselle Sánchez, Ricardo Bringas, Carlos Cordovés, Mario Valdés, Ricardo Lleonart, Luis Herrera, José de la Fuente, High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle. Journal of Biotechnology. ,vol. 33, pp. 135- 146 ,(1994) , 10.1016/0168-1656(94)90106-6
Steven L. Wagner, Robert S. Siegel, Thomas S. Vedvick, William C. Raschke, William E. Van Nostrand, High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursor Biochemical and Biophysical Research Communications. ,vol. 186, pp. 1138- 1145 ,(1992) , 10.1016/0006-291X(92)90865-I
Paul W. Howard, Richard A. Maurer, A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways. Journal of Biological Chemistry. ,vol. 270, pp. 20930- 20936 ,(1995) , 10.1074/JBC.270.36.20930