Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii.

摘要: Abstract The psbC gene of Chlamydomonas reinhardtii encodes P6, the 43 kd photosystem II core polypeptide. The sequence P6 is highly homologous to corresponding protein in higher plants with exception N-terminal region where first 12 amino acids are missing. Translation initiated at GUG C. reinhardtii. chloroplast mutant MA16 produces a unstable protein. mutation this strain maps near middle and consists 6 bp duplication that creates Ser-Leu repeat end one transmembrane domain. Two nuclear mutants, F34 F64, mutant, FuD34, unable synthesize P6. All these mutants accumulate wild-type levels mRNA. FuD34 has been localized 550 5' untranslated RNA can be folded into stem-loop structure. A suppressor isolated partially restores synthesis same region. These mutations appear define target site factor involved translation.