Cell signalling - the proteomics of it all.
作者： K. Ashman , M. F. Moran , F. Sicheri , T. Pawson , M. Tyers
DOI: 10.1126/STKE.2001.103.PE33
关键词: Computer science 、 Cell signaling 、 Scale (chemistry) 、 Computational biology 、 DNA microarray 、 Protein–protein interaction 、 Applied research 、 Proteomics 、 Nanotechnology 、 Protein Interaction Map
摘要: A challenge for biomedical scientists today is to arrive at an understanding of cellular behavior on a global scale. The advent DNA microarrays has greatly facilitated discovery gene expression profiles associated with different states. problem signaling the level interacting proteins in some ways more challenging. Ashman et al. discuss current methods available studying protein interactions scale, as well directions future. Technical hurdles exist many stages, from isolation complexes, determination their composition, software and databases needed analyze results large-scale, high-throughput datasets. suggest that, advances technology cooperation among academia industry, interaction map that underlies will emerge essential resource basic applied research.
sciencemag.org参考文章(44)
Howard R. Morris, Thanai Paxton, Maria Panico, Roy McDowell, Anne Dell, A Novel Geometry Mass Spectrometer, the Q-TOF, for Low-Femtomole/Attomole-Range Biopolymer Sequencing Journal of Protein Chemistry. ,vol. 16, pp. 469- 479 ,(1997) , 10.1023/A:1026309410737
Benno Schwikowski, Peter Uetz, Stanley Fields, A NETWORK OF PROTEIN?PROTEIN INTERACTIONS IN YEAST Nature Biotechnology. ,vol. 18, pp. 1257- 1261 ,(2000) , 10.1038/82360
Michael B Yaffe, German G Leparc, Jack Lai, Toshiyuki Obata, Stefano Volinia, Lewis C Cantley, None, A motif-based profile scanning approach for genome-wide prediction of signaling pathways Nature Biotechnology. ,vol. 19, pp. 348- 353 ,(2001) , 10.1038/86737
Huilin Zhou, Julian D. Watts, Ruedi Aebersold, A systematic approach to the analysis of protein phosphorylation Nature Biotechnology. ,vol. 19, pp. 375- 378 ,(2001) , 10.1038/86777
Stuart L. Schreiber, Gavin MacBeath, Printing proteins as microarrays for high-throughput function determination. Science. ,vol. 289, pp. 1760- 1763 ,(2000) , 10.1126/SCIENCE.289.5485.1760
Heng Zhu, Metin Bilgin, Rhonda Bangham, David Hall, Antonio Casamayor, Paul Bertone, Ning Lan, Ronald Jansen, Scott Bidlingmaier, Thomas Houfek, Tom Mitchell, Perry Miller, Ralph A Dean, Mark Gerstein, Michael Snyder, None, Global Analysis of Protein Activities Using Proteome Chips Science. ,vol. 293, pp. 2101- 2105 ,(2001) , 10.1126/SCIENCE.1062191
Susan E. Martin, Jeffrey Shabanowitz, Donald F. Hunt, Jarrod A. Marto, Subfemtomole MS and MS/MS Peptide Sequence Analysis Using Nano-HPLC Micro-ESI Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Analytical Chemistry. ,vol. 72, pp. 4266- 4274 ,(2000) , 10.1021/AC000497V
Alan G. Marshall, Christopher L. Hendrickson, George S. Jackson, Fourier transform ion cyclotron resonance mass spectrometry: A primer Mass Spectrometry Reviews. ,vol. 17, pp. 1- 35 ,(1998) , 10.1002/(SICI)1098-2787(1998)17:1<1::AID-MAS1>3.0.CO;2-K
Tony Pawson, John D Scott, Signaling Through Scaffold, Anchoring, and Adaptor Proteins Science. ,vol. 278, pp. 2075- 2080 ,(1997) , 10.1126/SCIENCE.278.5346.2075
John L Hartman IV, Barbara Garvik, Lee Hartwell, Principles for the Buffering of Genetic Variation Science. ,vol. 291, pp. 1001- 1004 ,(2001) , 10.1126/SCIENCE.291.5506.1001