Investigations in MALDI-MSI using a high repetition rate laser
作者： Rory Thomas Steven
DOI:
关键词: Tissue sections 、 Thin film 、 Maldi msi 、 Chemistry 、 Laser 、 Laser beams 、 Repeat analysis 、 Sampling (signal processing) 、 Maldi ms 、 Analytical chemistry
摘要: Within these studies the properties of a high repetition rate (≤ 25,000 Hz) Nd:YVO4 laser were investigated with view to improving throughput and quality information obtained from MALDI MS data collected in continuous raster sampling mode. Initially, nature beam profile was novel fluorometric method developed for imaging profiles. Analysis thin film samples α-cyano-4-hydroxycinnamic acid (CHCA) lipid standard phophatidylcholine (PC) 34:1 carried out. Under most conditions lower slow stage speed found be optimal. However, tissue based investigations rates 5-10 kHz faster speeds increase detected ion intensity. Subsequent this, use para-nitroaniline (PNA) as an effective matrix high-repetition MSI applications compared CHCA. PNA provide data, comparable or better than that The utility CHCA then repeat analysis single sections increasing amount obtained. Up five analyses same section demonstrated. Repeat applied acquisition both protein using multiple matrices washing.
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