Evaluation in situ of genotoxicity and stress in South American common toad Rhinella arenarum in environments related to fluorite mine.

摘要: Little attention has been paid to the impact of wastewater generated by mining activities on fluoride. In this study, we evaluated hematology responses common South American toad Rhinella arenarum inhabiting natural and artificial environments associated with a fluorite mine from central Argentina. We analyzed three sampling stations mine: (I) Los Cerros Negros stream (CN), which runs granitic rock high content; (II) Vallecitos (LV), metamorphic low (III) decantation ponds (DP) containing sediments produced flotation process. calculated frequencies micronuclei, erythrocyte nuclear abnormalities, mitosis, immature erythrocytes. addition, performed differential leukocyte count determined neutrophils/lymphocyte ratio as stress response estimator. found micronucleus (MN) abnormality (ENA) in DP CN but LV. The neutrophil/lymphocyte was different among sites, significant increase individuals DP. Values registered could be caused exposure mixture compounds dams that hold wastewater, while values might due concentrations Our results suggest blood is an effective non-destructive sensitive indicator for monitoring genotoxic agents freshwater ecosystems.