Phospholipid Signaling Protocols

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DOI: 10.1385/0896034917

关键词: ChemistryPhospholipasePhosphatidylinositolBiochemistryArachidonic acidSphingomyelinSphingosine kinaseCeramideDiacylglycerol kinasePhospholipase D activity

摘要: Part I. Monitoring of Activation Phospholipid-Derived Cell Signaling Pathways Phosphoinositidase C Assay I: Labeling, Stimulation, and Recovery Cellular [3H] Phosphoinositides Phosphoinositols Ian M. Bird II: Simple Analysis Recovered III: HPLC Measurement Using Radio High-Performance Liquid Chromatography Flow Detection Lubo Zhang Iain L. O. Buxton Preparation [3H]Phosphoinositol Standards Conversion to Inositol 1,4,5-Trisphosphate Mass Diacylglycerol Content Wendy Bollinger Bollag Richard D. Griner Phosphatidylinositol 4-Kinases: Assays Product Rachel E. Meyers Lewis C. Cantley Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) a Radioligand Displacement Jeroen van der Kaay, Pete J. Cullen, Peter Downes Phosphatidylinositol-4-Phosphate 5-Kinase Activity Thin-Layer Gregory Parker, Joost Loijens, A. Anderson Determination Phospholipase C- or D-Catalyzed Phosphatidylcholine Hydrolysis Michael Wakelam Trevor R. Pettitt D A2 in Cultured Cells Tritiated Arachidonic Acid Sandie Briand, Sylvie G. Bernier, Gaetan Guillemette Monoacylglycerol Lipases Bryan A.Wolf Isotopic Efflux Studies as Indices Jacob Vadakekalam Stewart Metz Extraction Measurements Prostanoids Leukotrienes by Radioimmunoassays Bruno Battistini, Serge Picard, Pierre Borgeat, Sirois Prostanoids, Leukotrienes, Isoprostanes By Enzyme Immunoassays Metabolites Derived from the Lipoxygenase High-Pressure Sphingomyelin Ceramide Levels After Sphingomyelinase-Mediated Pino Santana, Luisa F. Fanjul, Ruiz de Galarreta Galarreta, Fanjul Sphingosine Kinase: Ana Olivera Sarah Spiegel Analytical Methods Steps Sample For Molecular Species Fatty Acids Sanda Clejan for Separation In Phospholipids Sphingomyelins Ceramides II. General methods monitoring changes levels key signaling pathway proteins associated mRNA Solubilization Tissue Protein Cynthia Shaw Western Immunoblot Jing Zheng Immunohistochemistry Immunocytochemistry RNA Daniel S. Millican Size Quantification Northern

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