A molecular epidemiological investigation of Ascaris on Unguja, Zanzibar using isoenyzme analysis, DNA barcoding and microsatellite DNA profiling

摘要: Ascariasis is of public health importance on the islands Zanzibar (Unguja and Pemba). To shed light molecular epidemiology this parasite, 68 Ascaris worms, obtained from 14 individuals in four Ungujan villages, were examined by isoenzyme analysis (ISA), DNA barcoding microsatellite profiling. ISA revealed genetic variation, which was confirmed barcoding. Nineteen worms recovered Uganda included for comparison. Sixteen unique barcodes identified, 15 Unguja three with two shared between. These found all villages. Worms Tumbatu-Jongowe, an isolated village islet off Unguja, seemed particularly diverse. Within our barcodes, exact matches Chinese retrieved pigs, perhaps surprising given present rarity these animals Unguja. Microsatellite profiling population further diversity within samples although sub-structuring minor comparison to that between Uganda. As African has not been subjected detailed scrutiny, new represents important piece its evolutionary jigsaw such markers are informative monitoring worm dynamics during ongoing control.