Cellular and subcellular localization of S-adenosyl-L-methionine:benzoic acid carboxyl methyltransferase, the enzyme responsible for biosynthesis of the volatile ester methylbenzoate in snapdragon flowers.

摘要: The benzenoid ester, methylbenzoate is one of the most abundant scent compounds detected in majority snapdragon (Antirrhinum majus) varieties. It produced upper and lower lobes petals by enzymatic methylation benzoic acid reaction catalyzed S-adenosyl-L-methionine:benzoic carboxyl methyltransferase (BAMT). To identify location biosynthesis, we conducted an extensive immunolocalization study light electron microscopy at cellular subcellular levels using antibodies against BAMT protein. was immunolocalized predominantly conical cells inner epidermal layer and, to a much lesser extent, outer epidermis flower petal lobes. also located corolla tube with little protein yellow hairs within on bee's way nectar. These results strongly suggest that biosynthetic genes are expressed almost exclusively floral organs. Immunogold labeling studies reveal cytosolic enzyme, suggesting biosynthesis. concentration production surfaces face pollinators during landing may increase pollination efficiency help minimize cost advertising for pollinators.