In Vivo Imaging of Retinal and Choroidal Vasculature in the Rodent Eye using Optical Coherence Tomography

摘要: In vivo imaging technologies such as optical coherence tomography (OCT) and rodent models enable longitudinal studies of different ophthalmic pathologies their underlying physiological changes. this work a custom-made high resolution polarization sensitive OCT system is used to image the posterior eye small rodents like mice rats. Enface projections at certain depths/levels in three-dimensional (3D) reflectivity images visualize vascular structures retina well choroid. Further, phase resolved motion contrast based angiography allows create maps with distinguish between static tissue dynamic scatterers red blood cells. The levels are determined by semi-automatic approach which layers segmented first step. second step projection level can be adjusted operator restrict volume en-face carried out. To demonstrate functionality rats strains were imaged. anesthetized scanned an angle up 28° × 28°. Respiration induced artefacts compensated post-processing. on information variance showed that systems suitable resolve retinal capillaries outer plexiform layer. larger vessels within nerve fiber layer inner nuclear choroid visualized proposed approach.