PKS-NRPS Enzymology and Structural Biology: Considerations in Protein Production.
作者： Meredith A. Skiba , Finn P. Maloney , Qingyun Dan , Amy E. Fraley , Courtney C. Aldrich
DOI: 10.1016/BS.MIE.2018.01.035
关键词: Polyketide synthase 、 Nonribosomal peptide 、 Protein crystallization 、 Polyketide 、 Drug development 、 Structural biology 、 Chemistry 、 Protein purification 、 Protein folding 、 Computational biology
摘要: The structural diversity and complexity of marine natural products have made them a rich productive source new bioactive molecules for drug development. identification these compounds has led to extensive study the protein constituents biosynthetic pathways from producing microbes. Essential processes in dissection biosynthesis been elucidation catalytic functions determination 3D structures enzymes polyketide synthases nonribosomal peptide synthetases that carry out individual reactions. size proteins present numerous difficulties process going gene structure. Here, we review problems may be encountered at various steps this discuss some solutions devised our other labs cloning, production, purification, structure solution complex using Escherichia coli as heterologous host.
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