Single nucleotide polymorphism discovery in albacore and Atlantic bluefin tuna provides insights into worldwide population structure.

摘要: Summary The optimal management of the commercially important, but mostly over-exploited, pelagic tunas, albacore (Thunnus alalunga Bonn., 1788) and Atlantic bluefin tuna (BFT; Thunnus thynnus L., 1758), requires a better understanding population structure than has been provided by previous molecular methods. Despite numerous studies both species, their structures remain controversial. This study reports development single nucleotide polymorphisms (SNPs) in BFT application these SNPs to survey genetic variability across geographic ranges tunas. A total 616 werediscoveredin35albacoretunabycomparingsequencesof54nuclearDNAfragments.A panel 53 yielded FST values ranging from 0.0 0.050 between samples after genotyping 460 collected throughout distribution this species. No significant heterogeneity was detected within oceans, between-ocean comparisons (Atlantic, Pacific Indian oceans along with Mediterranean Sea) were significant. Additionally, 17-SNP developed cross-species amplification 107 fish. limited number discriminated two major spawning areas (FST = 0.116). SNP markers can be used genotype large numbers fish without need for standardizing alleles among laboratories.