Rolling circle amplification of DNA immobilized on solid surfaces and its application to multiplex mutation detection
作者： Anson Hatch , Takeshi Sano , John Misasi , Cassandra L. Smith
DOI: 10.1016/S1050-3862(98)00014-X
关键词: DNA ligase 、 Molecular biology 、 Biology 、 DNA 、 Rolling circle replication 、 DNA nanoball sequencing 、 Multiplex 、 Primer (molecular biology) 、 DNA polymerase I 、 Multiple displacement amplification
摘要: Abstract A new method of amplifying short DNA molecules immobilized on a solid support has been developed. This uses solid-phase rolling circle replication reaction, termed amplification (RCA). The probe consists single-stranded primer anchored at the 5′ terminus to and single stranded template hybridized primer. Here, ligase was used circularize template, polymerase I extend in reaction. identify known polymorphism BRCA1 exon 5. These results demonstrate that RCA offers considerable promise facilitate effective mutation screening using format.
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