Opposing chromatin remodelers control transcription initiation frequency and start site selection

摘要: Precise nucleosome organization at eukaryotic promoters is thought to be generated by multiple chromatin remodeler (CR) enzymes and affect transcription initiation. Using an integrated analysis of binding occupancy following rapid depletion, we investigated the interplay between these their impact on in yeast. We show that many are affected CRs operate concert or opposition position key start site (TSS)-associated +1 nucleosome. also movement after CR inactivation usually results from activity another absence any remodeling activity, nucleosomes largely maintain positions. Finally, present functional assays suggesting positioning often reflects a trade-off maximizing RNA polymerase recruitment minimizing initiation incorrect sites. Our provide detailed picture fundamental mechanisms linking promoter architecture