Urinary excretion of DNA repair products correlates with metabolic rates as well as with maximum life spans of different mammalian species.

摘要: Using recently developed methodology, which includes HPLC prepurification followed by GC/MS with isotope dilution, we analyzed urinary excretion of possible repair products oxidative DNA damage—8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), and 5-(hydroxymethyl)uracil (5—HMUra)-in mammalian species that substantially differ in metabolic rate longevity, namely, mice, rats, rabbits, dogs, pigs, humans. We found highly significant, positive correlations between specific rates the animals studied their for all modifications respective r values lesions (8-oxoGua) = .891, p < .01; (8-oxodG) .998, .001; (5-HMUra) .949, .005. However, only 8-oxoGua significantly correlates negatively maximum life span (MLSP) (r −.928, .01). Despite substantial differences MLSP humans pigs (120 27 years, respectively), measured were very similar. The levels our study mouse account respectively about 34,000 2800 repaired events per average cell, 24 h. It is therefore high mice (or other short-lived animals) may be responsible severe everyday insults accumulated faster than long-lived species.