Ultra Violet -B Induced DNA Damage in Human Leucocyte Cells
作者： Abhay Singh Yadav , Manoj Kumar Sharma
DOI: 10.1080/09723757.2008.11886042
关键词: Ultra violet 、 Comet tail 、 Comet assay 、 Gel electrophoresis 、 Irradiation 、 Cell 、 DNA damage 、 Dna instability 、 Biology 、 Molecular biology
摘要: The Single Cell Gel Electrophoresis (SCGE) Assay also known as Comet is a rapid, simple and visual technique for assessing DNA damage in individual cells. Effect of UV-B was studied by irradiating human leucocytes. Irradiation leucocyte increases the instability, which can be assessed Assay. blood samples were exposed to radiation various time period correlation found out between length comet tail exposure UV radiation. irradiation showed clear-cut dose effect relationship. It observed that with increase radiation, mean increased.
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