Amino acid composition of human uterine fluid: association with age, lifestyle and gynaecological pathology

摘要: studyquestion: Do the amino acid levels of human uterine fluid vary with age, BMI, phase menstrual cycle, benign pathology or diet? summaryanswer: The 18 acids in were shown to be affected only by maternal diet. what is known already: Murine, bovine and ovine content has been reported, but no reliable data on exist. Murine studies have demonstrated that intrauterine periconceptional nutritional environment study design, size, duration: Uterine secretions aspirated from 56 women aged –45 years. recruited preoperatively gynaecological theatre operating schedules hysterosalpingo-contrast-sonography (HyCoSy) lists. A proportion these had proven fertility; however, majority being investigated for subfertility. history dietary pattern also assessed. participants/materials, setting, methods: Reverse high performance liquid chromatography was used analyse concentrations within blood serum. results analysed against women’s stage BMI main role chance: profile described. In total, observed contain an concentration 3.54 mM (interquartile range: 2.27– 6.24 mM). relative not significantly altered cycle presence specific pathologies. However, a diet identified validated scoring system as less healthy associated higher asparagine (P ¼ 0.018), histidine 0.011), serine 0.033), glutamine 0.049), valine 0.025), phenylalanine 0.019), isoleucine 0.025) leucine 0.043) compared healthier diet, defined one intake fresh vegetables, fruit, whole-grain products fish low red processed meat fat dairy products. There significant correlations between serum those fluid. limitations, reasons caution: Our enabled us detect effect fluid; may sufficient numbers mild effects age. wider implications findings: These findings increase our understanding encountered preimplantation embryo, indicate how alter this. Given importance early embryo programming development future health, this information aid interventions aimed at optimizing vivo.