Protein and lipopolysaccharide profiles of a salt-sensitive Rhizobium sp. and its exopolysaccharide-deficient mutant.

摘要: Abstract A fast-growing, salt-sensitive rhizobium ( Rhizobium sp. ST1) with a narrow host range of infectivity was isolated from the root nodules locally grown pigeonpea Cajanus cajan ). One Tn5 mutants ST1 exopolysaccharide (EPS) deficient (exo − ) and showed 50% growth inhibition (GI 50 at 350 mM NaCl, compared to GI value wild type strain 250 mM NaCl. Whole cell protein profiles in presence NaCl an overall increase levels several proteins (22, 38, 68, >97 kDa), whereas its exo mutant, certain low molecular weight outer membrane (38 22 kDa) decreased. Other 40, 42,62 68 kDa) also markedly decreased both mutant salt. Similarly, lipopolysaccharide (LPS) components (LPS I LPS II) These observations suggest possible involvement components, along other factors, during under salt stress, relatively salt-tolerant strains rhizobia.