Characteristics of live parameters of the HS-5 human bone marrow stromal cell line cocultured with the leukemia cells in hypoxia, for the studies of leukemia-stroma cross-talk.

摘要: The unique bone marrow microenvironment is created by stromal cells and such physical conditions as hypoxia. Both hypoxia interactions with have a significant impact on the biology of leukemia cells, changing their sensitivity to antileukemic therapies. Thus, it crucial introduce biological systems, which enable investigation leukemia-stroma cross-talk verification novel therapies effectiveness under niche-mimicking conditions. Here, we established an experimental setup based hypoxic co-culture different cell lines derived from various patients. Flow cytometry enables simultaneous fluorescent tracking viable analysis fundamental cellular processes, also monitor basal vital state in co-culture. This critically important, deliver big variability signals protect provide drug resistance. Therefore, keeping at healthy during procedures. In proposed studies, viability, apoptosis, proliferation, ROS production, mitochondrial membrane potential were monitored both types, separated basis fluorescence tracker. We shown that do not affect live parameters indicating relevance model. Finally, utilized this stroma-mediated protection imatinib-induced death, contributes progression development therapy Altogether, recommend flow cytometric strategy elementary screen should be performed when using models. approach can broadly used for other studies part played leukemic screening studies.