Nitrogen-doped carbon nanocages and human umbilical cord mesenchymal stem cells cooperatively inhibit neuroinflammation and protect against ischemic stroke.

摘要: Abstract Aims This study aimed to explore the synergistic effects of nitrogen-doped carbon nanocages (NCNCs) and human umbilical cord mesenchymal stem cells (HUC-MSCs) on ischemic stroke investigate potential underlying mechanisms. Main methods The properties NCNCs were analyzed by transmission electron microscopy, markers HUC-MSCs detected flow cytometry. cell toxicity was evaluated MTT. Mice induced cerebral infarction transient middle artery occlusion (MCAO). or HUC-MSCs-NCNCs intravenously injected thirty minutes after reperfusion. infarct volume examined 2,3,5-triphenyltetrazolium chloride (TTC) staining, behavior tests modified Neurological Severity Score (mNSS) rotarod test. mRNA levels TNF-α IL-10 real-time PCR. protein stimulated gene/protein 6 (TSG-6) prostaglandin 2 (PGE2) ELISA. microglia (CD86 CD206) Iba1 CD16 determined immunostaining. Key findings enhanced therapeutic MCAO mice, including reducing volume, improving scores inhibiting inflammation response. In addition, cooperatively inhibit TNF-α, increased PGE2 TSG-6 in LPS-treated microglia. Furthermore, exerted with remodeling polarization. Significance enhance a mouse model, reactivation neuroinflammation, which indicates it as treatment for stroke.