Determination ofPenicillium roqueforti toxin by reversed-phase high-performance liquid chromatography

作者: Karin Siemens , Jerzy Zawistowski

DOI: 10.1016/0021-9673(92)80164-P

关键词: Phase (matter)Blue cheeseCoefficient of variationDetection limitPenicillium roquefortiHigh-performance liquid chromatographyToxinChromatographyChemistryReversed-phase chromatography

摘要: Abstract A method for the detection and quantification of Penicillium roqueforti toxin (PRT) using reversed-phase high-performance liquid chromatography has been established. The limit quantitation this was 3 ng PRT, while 2 toxin. precision analysis based on numerous runs good. Retention times PRT were highly reproducible with an average coefficient variation about 1.6%. Analysis in solid samples showed no interference sample matrix. accuracy 98.6%, mean recoveries 96.8%, 100.4% spiked culture medium blue cheese extracts, respectively.

参考文章(13)
S. Moreau, A. Masset, J. Biguet, Resolution of Penicillium roqueforti toxin and eremofortins A, B, and C by high-performance liquid chromatography. Applied and Environmental Microbiology. ,vol. 37, pp. 1059- 1062 ,(1979) , 10.1128/AEM.37.6.1059-1062.1979
Shenq-Chyi Chang, Yau-Huei Wei, Mei-Ling Liu, Ru-Dong Wei, Isolation and Some Properties of the Enzyme That Transforms Eremofortin C to PR Toxin Applied and Environmental Microbiology. ,vol. 49, pp. 1455- 1460 ,(1985) , 10.1128/AEM.49.6.1455-1460.1985
Ru-Dong Wei, Paul E. Still, E. B. Smalley, H. K. Schnoes, F. M. Strong, Isolation and Partial Characterization of a Mycotoxin from Penicillium roqueforti Applied Microbiology. ,vol. 25, pp. 111- 114 ,(1973) , 10.1128/AM.25.1.111-114.1973
S. Moreau, A. Lablache-Combier, J. Biguet, Production of Eremofortins A, B, and C Relative to Formation of PR Toxin by Penicillium roqueforti Applied and Environmental Microbiology. ,vol. 39, pp. 770- 776 ,(1980) , 10.1128/AEM.39.4.770-776.1980
YASUKO NAKAMURA, MINORU OHTA, YOSHIO UENO, Reactivity of 12, 13-Epoxytrichothecenes with Epoxide Hydrolase, Glutathione-S-Transferase and Glutathione Chemical & Pharmaceutical Bulletin. ,vol. 25, pp. 3410- 3414 ,(1977) , 10.1248/CPB.25.3410
Vladimír Betina, Thin-layer chromatography of mycotoxins. Journal of Chromatography A. ,vol. 334, pp. 211- 276 ,(1985) , 10.1016/S0021-9673(00)80272-1
Ru-dong Wei, H.K. Schnoes, P.A. Hart, F.M. Strong, The structure of PR toxin, a mycotoxin from Penicillium roqueforti Tetrahedron. ,vol. 31, pp. 109- 114 ,(1975) , 10.1016/0040-4020(75)85003-4
L. Polonelli, G. Morace, F. Delle Monache, R. A. Samson, STUDIES ON THE PR TOXIN OF PENICILLIUM ROQUEFORTI Mycopathologia. ,vol. 66, pp. 99- 104 ,(1978) , 10.1007/BF00429600