Interleukin-1α and -β modulation of luteinized human granulosa cell oestrogen and progesterone biosynthesis

摘要: Abstract This study was designed to test the hypothesis that interleukin-1 alpha (IL-1 alpha) and beta directly affect progesterone, oestradiol production in cultures of purified human granulosa cells. Luteinized cells were obtained from women during in-vitro fertilization cycles. Granulosa with without associated white blood cultured presence IL-1 (0.5-50 ng/ml) for 48 h. Media changed at 24 h intervals assayed progesterone oestradiol. In separate experiments, cell viability assessed tetrazolium salt reduction assay, haemocytometer counts, Trypan blue dye exclusion. Our results indicate synthesis by basal chorionic gonadotrophin (HCG)-stimulated co-cultured inhibited 5.0 ng/ml culture. cells, but not alpha. Oestradiol after both culture maximally affected beta. contrast, HCG-stimulated free only produced maximal inhibition (57%) (41%) Gonadal steroid mediated through cytotoxic or antiproliferative effects on Specificity response demonstrated abrogation anti-IL-1 neutralizing antibodies. conclusion, ovarian also exerted indirect via are usually present if steps taken remove them. These data support cytokines play an important role intra-ovarian regulation biosynthesis.