Succession and phylogenetic composition of bacterial communities responsible for the composting process of rice straw estimated by PCR-DGGE analysis

作者: Vila Ratri Cahyani , Kazuo Matsuya , Susumu Asakawa , Makoto Kimura

DOI: 10.1080/00380768.2003.10410052

关键词: 16S ribosomal RNAThermophilePhylogenetic treeBacteriaEcological successionCompostBotanyRice strawBiologyPcr dgge

摘要: Abstract PCR-DGGE analysis followed by sequencing of 16S rDNA was applied to reveal the succession and phylogenetic composition bacterial communities responsible for composting process rice straw (RS). RS under exhibited more complex DGGE band patterns with numerous bands diversification in intensity than initial materials. The materials were statistically divided into four groups, namely those characterizing associated pre-composting stage, thermophilic, middle, curing stages compost, which corresponded exactly same grouping obtained from PLFA pattern samples (Cahyani et al. 2002: Soil Sci. Plant Nutr., 48, 735-743). Different members characterized respective as follows: a-Proteobacteria materials, thermophilic Bacillus spp. actinomy...

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