Comparison of cellular effects of starch-coated SPIONs and poly(Lactic-co-glycolic acid) matrix nanoparticles on human monocytes

摘要: Within the last years, progress has been made in knowledge of properties medically used nanoparticles and their toxic effects, but still, little is known about influence on cellular processes immune cells. The aim our comparative study was to present two different nanoparticle types subcellular primary monocytes leukemic monocyte cell line MM6. We core-shell starch-coated superparamagnetic iron oxide (SPIONs) matrix poly(lactic-co-glycolic acid) (PLGA) for experiments. In addition typical biocompatibility testing like detection necrosis or secretion interleukins (ILs), we investigated impact these actin cytoskeleton voltage-gated potassium channels Kv1.3 Kv7.1. Induction not seen PLGA SPIONs MM6 Likewise, no alteration IL-1β IL-10 detected under same experimental conditions. contrast, IL-6 exclusively downregulated after contact with both nanoparticles. Two-electrode voltage clamp experiments revealed that reduce currents aforementioned channels. differed significantly cytoskeleton, demonstrated via atomic force microscopy elasticity measurement phalloidin staining. While led disruption respective did show any setups. difference effects ion suggests affect components pathways. Our data indicate effect are new parameters describe results highly relevant medical application further evaluation nanomaterial biosafety.