Hyperbranched mixed-mode anion-exchange polymeric sorbent for highly selective extraction of nine acidic non-steroidal anti-inflammatory drugs from human urine.

摘要: Abstract This paper describes the poly(divinylbenzene) (PDVB) supported synthesis of quaternized hyperbranched macromolecules (QHMs) and its use as a highly selective, high-capacity mixed-mode anion-exchange (MAX) sorbent. In detail, aminated PDVB support was firstly synthesized by copolymerization divinylbenzene 2-(diethylamino)ethyl methacrylate via Pickering emulsion polymerization. The QHMs were then grafted on divergent involving consecutive reactions resorcinol diglycidyl ether with methylamine (N, N-dimethylethanolamine for terminal epoxides), which brought in high density quaternary ammonium functionalities. changes specific surface area (SBET), pore volume ion exchange capacity (IEC) generation number reveal that have been grown successfully within large meso-channels porous PDVB. best compromise between SBET, IEC obtained at 4th (G4). Due to highest (0.47 meq/g), G4-QHMs applied solid phase extraction (SPE) acidic non-steroidal anti-inflammatory drugs (NSAIDs). An efficient approach based SPE coupled HPLC-UV developed selective cleanup nine NSAIDs (tolmetin, TLM; ketoprofen, KEP; naproxen, NAP; flurbiprofen, FLB; diclofenac, DIC; indomethacin, INM; ibuprofen, IBP; mefenamic acid, MFA; tolfenamic TFA) human urine samples. Under optimized conditions, method exhibited satisfactory recoveries ranging from 81.9% 104.0% relative standard deviation (RSD) values below 8.5%, good sensitivity (0.004–0.009 μg mL−1 limit detection) linearity (coefficient determination, R2 > 0.997, 0.01–0.2 μg mL−1 NAP, 0.05–1.0 μg mL−1 FLB, DIC, INM, MFA, TFA, 0.1–2.0 μg mL−1 TLM, KEP, IBP). MAX sorbent is superior Oasis HLB comparable obtaining clean chromatographic profiles. Our results demonstrate potential application complex sample analysis.