Substrate and sequence specificity of a eukaryotic DNA methylase
作者： Yosef Gruenbaum , Howard Cedar , Aharon Razin
DOI: 10.1038/295620A0
关键词: DNA clamp 、 Circular bacterial chromosome 、 DNA polymerase II 、 Biochemistry 、 Biology 、 In vitro recombination 、 DNA replication 、 Replication protein A 、 Eukaryotic DNA replication 、 Molecular biology 、 DNA supercoil
摘要: In eukaryotic DNA, 50–90% of the dinucleotide sequence C-G is methylated. Most methylated sites are apparently placed at fixed locations in genome and this methylation pattern faithfully inherited from generation to generation1. Holliday Pugh2 Riggs3 have suggested that methyl moieties a semi-conservative fashion during DNA replication, model has been confirmed by experiments which was integrated into mouse L-cells following DNA-mediated gene transfer4–6. For mechanism operate, two basic requirements must be satisfied: (1) symmetrically on both strands DNA7,8 (2) cellular methylase should specific for hemi-methylated substrate present replication. Here we demonstrate conclusively preferred vitro ascites indeed DNA. Furthermore, enzyme seems methylate exclusively cytosine residues located
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