Silencing of HIF-1α by RNA Interference in Human Glioma Cells In Vitro and In Vivo
作者： David L. Gillespie , Jeannette R. Flynn , Brian T. Ragel , Maria Arce-Larreta , David A. Kelly
DOI: 10.1007/978-1-60327-547-7_14
关键词: RNA interference 、 Vascular endothelial cell proliferation 、 In vivo 、 Cancer research 、 DNA-directed RNA interference 、 Chemistry 、 Gene silencing 、 Glioma 、 Vascular endothelial growth factor 、 Hypoxia (medical)
摘要: Higher-grade gliomas are distinguished by increased vascular endothelial cell proliferation and peritumoral edema. These thought to be instigated growth factor, which in turn is regulated cellular oxygen tension. Hypoxia inducible factor-1alpha (HIF-1alpha) a main responder intracellular hypoxia overexpressed many human cancers, including gliomas. Here we present methods for investigating the role of HIF-1alpha glioma vivo vitro using RNA interference U251, U87, U373 cells.
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