Genotyping and phenotyping of Fusarium graminearum isolates from Germany related to their mycotoxin biosynthesis.

摘要: Abstract Fusarium graminearum is the most important pathogen causing head blight (FHB) of small cereal grains worldwide responsible for quantitative and qualitative yield losses. The presence in crops often associated with mycotoxin contamination foodstuff limiting its use human animal consumption. A collection isolates F. from Germany was characterized genetically chemically their potential to produce B trichothecenes deoxynivalenol (DON) nivalenol (NIV). Molecular methods eight PCR assays were implemented based on functional Tri7 Tri13 genes tri5–tri6 intergenic region differentiate between chemotaxonomic groups DON NIV, resulting a marked majority (61/63) chemotypes. Mycotoxins produced rice kernels quantified by means LC-MSMS including DON, 3-acetyl-DON (3-ADON), 15-acetyl-DON (15-ADON), DON-3-glucoside, fusarenon X, as well zearalenone; all them proving be present high concentration among isolates. All DON-chemotype also lower amounts NIV amount being positively correlated (R² = 0.89) amount. 15-ADON 3-ADON are reported simultaneously isolates, former dominating over latter but one isolate. Fungal biomass, via ergosterol rice. It used calculate specific production per biomass ranging 0.104 1.815 mg mg-1 ergosterol, presenting Gaussian distribution. Genotype phenotype characterization revealed discrepancies respect fungi, i.e. chemotype able mycotoxins other chemotypes considerable amounts.