Novel lipidic and bienzymatic nanosomes for efficient delivery and enhanced bioactivity of catalase.
作者： Yao Li , Yunli Zhou , Wenli Han , Mingxin Shi , Hua Zhao
DOI: 10.1016/J.IJPHARM.2017.09.006
关键词: Biochemistry 、 Nanocarriers 、 Bioavailability 、 Chemistry 、 Biological membrane 、 Catalase 、 Peroxidase 、 Normal level 、 In vivo 、 Hydrogen peroxide 、 Chromatography
摘要: Abstract The purpose of this study was to evaluate the improved characteristics catalase (CAE) when loaded in lipidic and bienzymatic nanosomes. Lipidic nanosomes containing CAE uricase (LSCU) were manufactured two buffer solutions. Their micromorphologies, sizes, zeta potentials, enzymatic activities, kinetic characteristics, hydrogen peroxide-lowering effects compared with those free only (LSC). structural change stability mechanism investigated using fluorescent probes. Compared LSC, LSCU had better physiochemical vitro activity under different temperatures pH conditions. In vivo bioavailability peroxidase also improved. For example, ∼450% greater than that CAE, time required for lower peroxide concentrations a physiologically normal level almost one-third one-half LSC. increased catalytic capabilities could be ascribed favorable conformational changes protection offered by nanosomal biomembrane. might promising nanocarriers parenteral delivery therapeutic enzymes such as CAE.
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