Screening for lead compounds and herbal extracts with potential anti-influenza viral activity.
作者： Kiattawee Choowongkomon , Pongrama Ramasoota , Chalermpol Lekcharoensuk , Nantawan Petcharat , Porntippa Leckcharoensuk
DOI:
关键词: Infectivity 、 Influenza A virus 、 Virtual screening 、 Electrophoretic mobility shift assay 、 Binding domain 、 In vitro 、 Biology 、 Biochemistry 、 RNA silencing 、 RNA
摘要: Nonstructural protein 1 (NS1) of the highly pathogenic avian influenza virus (H5N1) contains a conserved RNA binding domain (RBD) that inhibits antiviral functions host-innate immune response. Dimerization NS1 forms central groove and binds to double stranded (ds) RNA. This region might serve as potential drug target. In this study, three dimensional structure model RBD was constructed virtual screening performed identify lead compounds bound within around groove. The showed 5 with energy ranging between -16.05 -17.36 Kcal/mol. Two commercially available compounds, estradiol veratridine, were selected for using in an vitro assay. results neither could inhibit association dsRNA protein. addition, 34 herbal extracts examined their inhibitory effects. Five them able electrophoresis mobility shift Four herbs, Terminalia belirica, Salacia chinensis, Zingiber montanum Peltophorum pterocarpum, reduce > 50% infectivity H5N1 cell-based assay, it is worth further studying use source drugs.
参考文章(37)
Jiyong Zhou, Wenbo Sun, Junhua Wang, Junqing Guo, Wei Yin, Nanping Wu, Lanjuan Li, Yan Yan, Ming Liao, Yu Huang, Kaijian Luo, Xuetao Jiang, Hualan Chen, None, Characterization of the H5N1 Highly Pathogenic Avian Influenza Virus Derived from Wild Pikas in China Journal of Virology. ,vol. 83, pp. 8957- 8964 ,(2009) , 10.1128/JVI.00793-09
Leslie A. Reperant, Marco W. G. van de Bildt, Geert van Amerongen, Debbie M. Buehler, Albert D. M. E. Osterhaus, Susi Jenni-Eiermann, Theunis Piersma, Thijs Kuiken, Highly Pathogenic Avian Influenza Virus H5N1 Infection in a Long-Distance Migrant Shorebird under Migratory and Non-Migratory States PLoS ONE. ,vol. 6, pp. e27814- 27814 ,(2011) , 10.1371/JOURNAL.PONE.0027814
G. Boivin, N. Goyette, H. Bernatchez, Prolonged Excretion of Amantadine-Resistant Influenza A Virus Quasi Species after Cessation of Antiviral Therapy in an Immunocompromised Patient Clinical Infectious Diseases. ,vol. 34, ,(2002) , 10.1086/338870
Hongbo Zhou, Jiping Zhu, Jiagang Tu, Wei Zou, Yong Hu, Zhengjun Yu, Wensi Yin, Yongtao Li, Anding Zhang, Yurong Wu, Ziniu Yu, Huanchun Chen, Meilin Jin, Effect on Virulence and Pathogenicity of H5N1 Influenza A Virus through Truncations of NS1 eIF4GI Binding Domain The Journal of Infectious Diseases. ,vol. 202, pp. 1338- 1346 ,(2010) , 10.1086/656536
Zejun Li, Yongping Jiang, Peirong Jiao, Aiqin Wang, Fengju Zhao, Guobin Tian, Xijun Wang, Kangzhen Yu, Zhigao Bu, Hualan Chen, The NS1 Gene Contributes to the Virulence of H5N1 Avian Influenza Viruses Journal of Virology. ,vol. 80, pp. 11115- 11123 ,(2006) , 10.1128/JVI.00993-06
Siri H. Hauge, Susanne Dudman, Katrine Borgen, Angie Lackenby, Olav Hungnes, Oseltamivir-resistant influenza viruses A (H1N1), Norway, 2007-08. Emerging Infectious Diseases. ,vol. 15, pp. 155- 162 ,(2009) , 10.3201/EID1502.081031
Thomas W. Vahlenkamp, Timm C. Harder, Matthias Giese, Fengsheng Lin, Jens P. Teifke, Robert Klopfleisch, Ralf Hoffmann, Ian Tarpey, Martin Beer, Thomas C. Mettenleiter, Protection of cats against lethal influenza H5N1 challenge infection. Journal of General Virology. ,vol. 89, pp. 968- 974 ,(2008) , 10.1099/VIR.0.83552-0
Garrett M. Morris, David S. Goodsell, Robert S. Halliday, Ruth Huey, William E. Hart, Richard K. Belew, Arthur J. Olson, Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function Journal of Computational Chemistry. ,vol. 19, pp. 1639- 1662 ,(1998) , 10.1002/(SICI)1096-987X(19981115)19:14<1639::AID-JCC10>3.0.CO;2-B
Dipanwita Basu, Marcin P. Walkiewicz, Matthew Frieman, Ralph S. Baric, David T. Auble, Daniel A. Engel, Novel influenza virus NS1 antagonists block replication and restore innate immune function. Journal of Virology. ,vol. 83, pp. 1881- 1891 ,(2009) , 10.1128/JVI.01805-08
A. J. Hay, M. C. Zambon, A. J. Wolstenholme, J. J. Skehel, M. H. Smith, Molecular basis of resistance of influenza A viruses to amantadine. Journal of Antimicrobial Chemotherapy. ,vol. 18, pp. 19- 29 ,(1986) , 10.1093/JAC/18.SUPPLEMENT_B.19