Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes
作者： Dayle A. Hager , Richard R. Burgess
DOI: 10.1016/0003-2697(80)90013-5
关键词: RNA polymerase 、 Sodium 、 Guanidine 、 Sodium dodecyl sulfate 、 Chromatography 、 Chemistry 、 Biochemistry 、 Sigma factor 、 Escherichia coli 、 Alkaline lysis 、 Polyacrylamide gel electrophoresis
摘要: … electrophoresis step (I), lengthy dialysis, or the use of SDS … buffer compositions was also seen with the other polymerase subunits. For this reason, the buffer containing 0.1% SDS …
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