Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme.
作者： María J Mazón , Juana M Gancedo , Carlos Gancedo , None
DOI: 10.1016/S0021-9258(19)68160-3
关键词: Fructose 1,6-bisphosphatase 、 Sodium dodecyl sulfate 、 Enzyme assay 、 Serine 、 Enzyme 、 Chemistry 、 Yeast 、 Molecular biology 、 Phosphate 、 Biochemistry 、 Enzyme Reactivation
摘要: Incorporation of 32P into yeast fructose-1,6-bisphosphatase (EC 3.1.3.11) was observed after addition glucose to a cell suspension incubated with (32P)orthophosphoric acid. The counts were coincident the enzyme band when immunoprecipitates subjected sodium dodecyl sulfate disc gel electrophoresis. incorporation phosphate associated decrease in activity. Approximately 1 mol incorporated/mol enzyme. is bound phosphoester linkage serine residue. Release accompanying reactivation both vivo and cell-free extracts.
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