Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi.

摘要: The ability of decreasing inocula Borrelia burgdorferi to grow in otherwise identical Barbour-Stoenner-Kelly (BSK) media containing different lots bovine serum albumin (fraction V) was determined. These differed significantly detect B. burgdorferi. Some BSK required 2 x 10(5) organisms per ml for detection, while other could stimulate growth after inoculation with less than ml. In addition, from the sensitive were thinner, longer, and tightly coiled. endpoint dilutions indirect fluorescent-antibody titers, especially immunoglobulin M, exhibited up 16-fold decreases, both G M titers more difficult interpret diagnostic slides prepared some thinner results demonstrate that, when performing laboratory investigations which rely on burgdorferi, it is essential that quality medium be