13 C-metabolic flux analysis and its applications
作者： Kazuyuki Shimizu
DOI: 10.1533/9781908818201.263
关键词: Pentose phosphate pathway 、 Escherichia coli 、 Flux (metabolism) 、 Cyanobacteria 、 Metabolic flux analysis 、 Biology 、 Heterotroph 、 Synechocystis 、 Biochemistry 、 Metabolite
摘要: The basic idea and the algorithm for 13 C metabolic flux analysis are explained. Its application to wild-type Escherichia coli using glucose or acetate as a carbon source is explained based on isotopomer data obtained by GC-MS. Other applications of pckA, pgi , zwf gene knockout E. strains NMR. By combining information available from NMR, GC-MS, metabolite balances, intracellular distributions in Synechocystis grown under heterotrophic mixotrophic conditions can be quantified, where pentose phosphate pathway major catabolism culture, which switched Calvin cycle when light supplied cyanobacterial cells. operation 4 cells identified, labeling data.
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