Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles
作者： Christopher J Roberts , Bryce Nelson , Matthew J Marton , Roland Stoughton , Michael R Meyer
DOI: 10.1126/SCIENCE.287.5454.873
关键词: Genetics 、 Cell biology 、 Fus3 、 Signal transduction 、 Gene 、 Gene expression 、 Transcription factor 、 Gene expression profiling 、 Fungal protein 、 Ste5 、 Biology
摘要: Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 the most highly regulated genes, constructed from 46 diverse experimental conditions. Diagnostic subsets coexpressed genes reflected signaling activity, cross talk, overlap multiple mitogen-activated protein kinase (MAPK) pathways. Analysis profiles specified by two different MAPKs-Fus3p Kss1p-revealed functional filamentous growth mating responses. Global reflects biological responses associated with activation perturbation
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