Expression and clinical significance of ezrin mRNA in human primary sporadic colorectal carcinoma

摘要: Objective To investigate the expression pattern of membrane-cytoskeleton linker ezrin mRNA in human sporadic colorectal carcinoma （SCRC） , and to evaluate correlation with carcinogenesis, progression metastasis SCRC. Methods TaqMan real-time reverse transcription polymerase chain reaction （ RT-PCR） technique immunohistochemical IHC ）. EnVision staining were used detect protein 132 SCRC specimens 43 adjacent normal mucosa specimens, including 74 cases 58 without. The between process carcinogenesis was analyzed, location clinicopathological parameters also analyzed. Results rate significantly higher than that （P 〈 0.05）; which metastatic group non-metastatic （2. 3580 ± 0.5584 vs. 2.1129 0.6625, P = 0.037 ;86.5% 70.7%, 0. 026）. on membrane 31.1% versus 6.9% 0.001 ）, but only slightly 2671 0.5990） 2448 ±0. 6241 No significant difference found cytoplasmic （55.4%） （63. 8% Ezrin at age 50 years （2.5165 5755 ） ≥ 1762 6094, 016）. right colon left rectum 0003 rectum（ 004 level age, sex, tumor size, location, differentiation depth invasion. Conclusion expressions closely correlated SCRC, is consistent trend mRNA. switching localization from cytoplasm may play an important role metastasis. patients＇age; membrane, related location. potentially become parameter determining biological behavior SCRC. Key words: Colorectal neoplasms ;  Ezrin;  Immunohistochemistry Real- time RT-PCR