Changes of rat testicular germ cell apoptosis after high power microwave radiation
作者： Chunhua Yu , Ying Yang , Donghong Li , Yuanqing Yao
DOI:
关键词: Microwave 、 Germ cell 、 Rats sprague dawley 、 Chemistry 、 Apoptosis 、 Andrology 、 TUNEL assay 、 End labeling 、 Testicular germ cell
摘要: OBJECTIVE: To study the effect of high power microwave (HPM) radiation on testicular germ cell apoptosis. METHODS: One hundred and twenty-five Spraque-Dawley rats were randomly divided into two groups, unexposed control group experimental group(further four subgroups: 10 mW/cm2 5 min, 20 mw/cm2 min), then was radiated with S wave band mW/cm2, for or min. Testicular samples taken at 6 h, 24 48 72 h d after separately studied. At end process, apoptosis detected by in situ terminal deoxynucleotityl transferase mediated dUTP nick labeling (TUNEL). RESULTS: The number apoptotic cells groups min remarkably larger than that controls (P 0.05). CONCLUSION: HPM can increase rat testis, which is related to time sample acquisition. In condition present test, minutes may significantly enhance damage, turn influence reproductive function rats.
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