Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in a Light-Dependent Manner.
作者： A. Leyva , J. A. Jarillo , J. Salinas , J. M. Martinez-Zapater
DOI: 10.1104/PP.108.1.39
关键词: Reporter gene 、 Mutant 、 Phenylpropanoid 、 Light intensity 、 Biochemistry 、 Phenylalanine ammonia-lyase 、 Arabidopsis 、 Chalcone synthase 、 Biology 、 Arabidopsis thaliana
摘要: Anthocyanins, which accumulate in leaves and stems response to low temperature changes light intensity, are synthesized through the phenylpropanoid pathway that is controlled by key enzymes include phenylalanine ammonia-lyase (PAL) chalcone synthase (CHS). In this work we demonstrate PAL CHS mRNAs of Arabidopsis thaliana (L.) Heynh. upon exposure a light-dependent manner. The regulation PAL1 gene expression was examined analyzing [beta]-glucuronidase (uidA) reporter transgenic plants containing uidA Escherichia coli under control promoter. results indicate accumulation mRNA transcriptionally regulated. Histochemical staining for activity showed promoter preferentially activated photosynthetically active cells, paralleling anthocyanin accumulation. Moreover, show may also be implicated bacterial infiltration. Finally, using two transparent testa mutants unable anthocyanins, these pigments not required successful development freezing tolerance species.
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S. Kiedrowski, P. Kawalleck, K. Hahlbrock, I.E. Somssich, J.L. Dangl, Rapid activation of a novel plant defense gene is strictly dependent on the Arabidopsis RPM1 disease resistance locus The EMBO Journal. ,vol. 11, pp. 4677- 4684 ,(1992) , 10.1002/J.1460-2075.1992.TB05572.X
A. H. Teramura, E. M. Middleton, The Role of Flavonol Glycosides and Carotenoids in Protecting Soybean from Ultraviolet-B Damage. Plant Physiology. ,vol. 103, pp. 741- 752 ,(1993) , 10.1104/PP.103.3.741
J. Levitt, Responses of Plants to Environmental Stress, 2nd Edition, Volume 1: Chilling, Freezing, and High Temperature Stresses. Responses of Plants to Environmental Stress, 2nd Edition, Volume 1: Chilling, Freezing, and High Temperature Stresses.. ,(1980)
R. A. Jefferson, T. A. Kavanagh, M. W. Bevan, GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal. ,vol. 6, pp. 3901- 3907 ,(1987) , 10.1002/J.1460-2075.1987.TB02730.X
Jeffery L Dangl, None, Regulatory Elements Controlling Developmental and Stress-induced Expression of Phenylpropanoid Genes Springer, Vienna. pp. 303- 326 ,(1992) , 10.1007/978-3-7091-6684-0_12
Yoshiyuki TANAKA, Ikuzo URITANI, Purification and Properties of Phenylalanine Ammonia-Lyase in Cut-injured Sweet Potato Journal of Biochemistry. ,vol. 81, pp. 963- 970 ,(1977) , 10.1093/OXFORDJOURNALS.JBCHEM.A131562
Isaac Rabino, Alberto L. Mancinelli, Light, Temperature, and Anthocyanin Production Plant Physiology. ,vol. 81, pp. 922- 924 ,(1986) , 10.1104/PP.81.3.922
Thomas W. Kimmerer, Theodore T. Kozlowski, Ethylene, Ethane, Acetaldehyde, and Ethanol Production By Plants under Stress Plant Physiology. ,vol. 69, pp. 840- 847 ,(1982) , 10.1104/PP.69.4.840
Sarah J. Gilmour, Ravindra K. Hajela, Michael F. Thomashow, Cold Acclimation in Arabidopsis thaliana Plant Physiology. ,vol. 87, pp. 745- 750 ,(1988) , 10.1104/PP.87.3.745
Michael J.C. Rhodes, Leonard S.C. Wooltorton, Changes in the activity of enzymes of phenylpropanoid metabolism in tomatoes stored at low temperatures Phytochemistry. ,vol. 16, pp. 655- 659 ,(1977) , 10.1016/S0031-9422(00)89225-0