MICROTUBULE DYNAMICS IN OXIDATIVELY-STRESSED NEURONAL CELLS

摘要: The microtubule (MT) system is important for many aspects of neuronal function, including motility, differentiation, and organelle trafficking. dysregulation this can therefore have a significant impact on function survival. Parkinson’s disease (PD) associated with alterations in integrity the axon/dendrites as well axonal transport, which suggestive altered MT function. In fact, recent studies using genetic toxin models are beginning to implicate dysfunction key mechanism underlying degeneration PD. To further study role PD neurodegeneration, effects oxidative stress, plays pathogenesis, were examined commonly utilized toxin, 6-hydroxydopamine (6OHDA). response 6OHDA-induced stress cells, observed reductions growth rate, increase frequency pauses/retractions, impaired end binding protein 1 (EB1) levels, levels tubulin acetylation. Impaired deacetylases, specifically sirtuin 2 (SIRT2), was oxidatively-stressed cells. Restoration deacetylase rescued neuritic phenotype 6OHDA, suggesting that impairs by altering addition its neurite, disruption also affected MT-dependent nuclear could contribute selective declines transcriptional responses diseased dopaminergic neurons. all, provides support degenerative processes seen Elucidating how stress-induced changes lead will provide insight into development novel therapeutic strategies.