Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II.
作者： Manzoor A Bhat , Alastair Valentine Philp , David M Glover , Hugo J Bellen
DOI: 10.1016/S0092-8674(00)81804-8
关键词: Cyclin B 、 Biology 、 Mitosis 、 Centromere 、 Condensin 、 Anaphase 、 Chromatin 、 Molecular biology 、 Sister chromatids 、 Mitotic chromosome condensation
摘要: We have isolated a Drosophila gene, barren (barr), required for sister-chromatid segregation in mitosis. barr encodes novel protein that is present proliferating cells and has homologs yeast human. Mitotic defects embryos become apparent during cycle 16, resulting loss of PNS CNS neurons. Centromeres move apart at the metaphase-anaphase transition Cyclin B degraded, but sister chromatids remain connected, chromatin bridging. This phenotype similar to described TOP2 mutants yeast. Barren localizes throughout Colocalization biochemical experiments indicate associates with Topoisomerase II mitosis alters activity II. propose this association proper chromosomal by facilitating decatenation anaphase.
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