Dichotomic Phylogenetic Tree of the Pyruvate Kinase Family K+-DEPENDENT AND -INDEPENDENT ENZYMES

摘要: K+ dependence was assumed to be a feature of all pyruvate kinases until it discovered that some enzymes express -independent activity. Almost the have Lys at position 117, instead Glu present in -dependent muscle enzyme. Mutagenesis studies show internal positive charge substitutes for requirement (Laughlin, L. T. & Reed, G. H. (1997) Arch. Biochem. Biophys. 348, 262-267). In this work phylogenetic analysis kinase performed ascertain abundance activities and explore whether activating effect is related evolutionary history Of 230 studied sequences, 46% rest Glu. Pyruvate with Lys117 Glu117 are separated two clusters. All cluster been characterized -dependent, whereas those -independent. Thus, there strict correlation between dichotomy tree activity on K+. 77% possess Lys113/Gln114; they also Ile, Val, or Leu 120. These residues replaced by Thr113/Lys114/Thr120 80% kinases. Structural indicates these hinge region involved acquisition catalytic conformation The route conversion from described. A plausible explanation how developed put forth.