Organic amendments change soil organic C structure and microbial community but not total organic matter on sub-decadal scales

摘要: Abstract Organic C has many benefits for soil, but it is depleted by tillage and crop harvest, especially so biofuel crops. Accordingly, strategies such as partially retaining stover or planting a cover can help ameliorate the negative effect of removal. We used long-term field experiment to study impacts retention on soil organic matter (SOM), its extractable components, microbial community. SOM chemical composition characterization was determined electrospray ionization (ESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) in sequential water, methanol (MeOH), chloroform (CHCl3) extracts. The characteristics bacterial community were measured phospholipid fatty acid (PLFA), real-time quantitative PCR, 16S rRNA gene sequence. variations total content, biomass, population slight among treatments, compounds, arbuscular mycorrhizal fungi (AMF) structure changed significantly, application crop. Specifically, enriched more lignin-like compounds whereas condensed hydrocarbons, had an aromaticity index (AI) > 0.5. not altered crop, corn increased relative abundances Myxococcales (Deltaproteobacteria) decreased that Actinobacteria. Redundancy analysis (RDA) further revealed treatments significant positive association CHCl3-extracted classes, i.e. unsaturated hydrocarbons lipids, (stover crop), lignin proteins only treatment. Taken together, our shows how different addition practices influence molecular communities.