High-sensitivity nucleic acid preparation methods for the detection of nucleic acid by nucleic acid polymerase
作者： Sora Choi , Hyun Seo Kim , Jun Hee Lee , Jong Hoon Kim , Han Oh Park
DOI:
关键词: Nucleic acid quantitation 、 Terminator (genetics) 、 Nucleic acid methods 、 Biochemistry 、 Nucleic acid 、 Real-time polymerase chain reaction 、 Nucleic acid thermodynamics 、 Polymerase 、 Polymerase chain reaction 、 Chemistry
摘要: The present invention relates to a method for preparing nucleic acid with high sensitivity, wherein polymerase is used add terminator the be analysis prior polymerization reaction such as PCR reaction, real time quantitative or like, detecting trace of acid, that non-specific priming occurring competitively an amplification target may basically eliminated, thereby precisely only and measuring concentration acid.
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