A simplified microassay of DNA and RNA using ethidium bromide
作者： G.J. Boer
DOI: 10.1016/0003-2697(75)90507-2
关键词: RNA 、 Freeze-drying 、 Ethidium bromide 、 Detection limit 、 Tissue sections 、 Biochemistry 、 DNA 、 Pronase 、 Ethidium homodimer assay 、 Biology 、 Biophysics 、 Cell biology 、 Molecular biology
摘要: Abstract The ethidium bromide method of Karsten and Wollenberger (3) has been modified allowing determination DNA RNA in small samples tissue homogenates (10–30 μg wet weight) or pieces frozen-dried sections (2–5 dry weight). detection limit for ranges between 5 10 ng, depending on the origin tissue, is twice as high. present micromethod offers a good fluorimetric alternative more laborious diaminobenzoic acid assay Kissane Robins (4).
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sci-hub.se 参考文章(7)
J.-B. Lepecq, C. Paoletti, A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. Journal of Molecular Biology. ,vol. 27, pp. 87- 106 ,(1967) , 10.1016/0022-2836(67)90353-1
John M. Kissane, Eli Robins, The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry. ,vol. 233, pp. 184- 188 ,(1958) , 10.1016/S0021-9258(19)68051-8
Mary J. Blackburn, T.M. Andrews, R.W.E. Watts, Measurement of DNA in isolated granulocytes by the ethidium technique Analytical Biochemistry. ,vol. 51, pp. 1- 10 ,(1973) , 10.1016/0003-2697(73)90446-6
Uwe Karsten, Albert Wollenberger, Determination of DNA and RNA in homogenized cells and tissues by surface fluorometry Analytical Biochemistry. ,vol. 46, pp. 135- 148 ,(1972) , 10.1016/0003-2697(72)90405-8
OLIVER H. LOWRY, The quantitative histochemistry of the brain; histological sampling. Journal of Histochemistry and Cytochemistry. ,vol. 1, pp. 420- 428 ,(1953) , 10.1177/1.6.420
G. J. Bohr, J. F. Jongkind, Quantitative lysosomal enzyme activity changes in the neural lobe of the rat following water deprivation and lactation. Journal of Neurochemistry. ,vol. 22, pp. 965- 970 ,(1974) , 10.1111/J.1471-4159.1974.TB04323.X
Jean-Bernard Le Pecq, Claude Paoletti, A new fluorometric method for RNA and DNA determination Analytical Biochemistry. ,vol. 17, pp. 100- 107 ,(1966) , 10.1016/0003-2697(66)90012-1