Influence of growth and environmental conditions on cell surface hydrophobicity of Pseudomonas fluorescens in non-specific adhesion

作者: T K Jana , A K Srivastava , K Csery , D K Arora

DOI: 10.1139/W99-104

关键词: AdhesionPseudomonadalesPseudomonadaceaeGrowth mediumMicrobiologyProteinase KHydrophilic interaction chromatographyFood scienceMacrophomina phaseolinaBiologyPseudomonas fluorescens

摘要: The relative cell surface hydrophobicity (CSH) of 18 soil isolates Pseudomonas fluorescens, determined by phase exclusion, hydrophobic interaction chromatography (HIC), electrostatic (ESIC), and contact angle, revealed large degrees variability. Variation in the adhesion efficiency to Macrophomina phaseolina hyphae/sclerotia these was also examined. Two such with maximum (32.8%; isolate 12-94) minimum (12%; 30-94) CSH were selected for further study. Early- mid-log exponential cells more than those stationary phase, influenced fluctuations temperatures pH. Isolate 12-94 exhibited high (32.3%) at 30°C, compared lower values (28-24%) higher temperature range (35-40°C). Increasing concentrations either Zn2+, Fe3+, K+, Mg2+ growth medium associated increased CSH. Trypsin, pepsin, proteinase K (75 150 ...

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