Analysis of the role of MHC class II presentation in the stimulation of cytotoxic T lymphocytes by antigens targeted into the exogenous antigen-MHC class I presentation pathway.

摘要: By conjugation of proteins to beads, Ags can be selectively targeted into the MHC class I pathway phagocytes in vivo and stimulate CTL responses. Because also present particulate Ag on II molecules, we examined whether these stimulated concomitant CD4 T cell immunity. Although priming cells with soluble OVA required adjuvants, was stimulatory when injected saline. We next responses played a role generation Ag. At low concentrations Ag, primed CTLs wild-type mice but not II-deficient animals, indicating that presentation essential for generation. These data both support model where collaborate as part three-cell interaction identify phagocyte third this reaction. Interestingly, injection higher same equivalent mice. results indicate key variable determining is helper dependent or independent dose immunogen. This may explain why abundant Ags, such viruses, tend independent, while less minor histocompatibility require cells. In addition, point potential using prime boost settings immunodeficiency.