Hepatoprotective and antioxidant effects of Myelophil on restraint stress-induced liver injury in BALB/c mice

摘要: Abstract Ethnopharmacologic relevance Myelophil, an ethanol extract of Astragali Radix and Salviae Radix, has been used for patients with chronic fatigue-associated disorders in traditional Oriental clinics. Aim the study This aimed to evaluate pharmacologic effects Myelophil using a restraint stress-induced liver injury model explore mechanisms underlying these effects. Materials methods Distilled water or (50 mg/kg, 100 mg/kg, 200 mg/kg) was orally administered 6-week-old BALB/c male mice once daily 5 day. The were subjected stress 6 h, serum levels aspartate alanine aminotransferases (AST ALT, respectively), total reactive oxygen species, antioxidant capacity determined. Hepatic tissue lipid peroxidation, activity (as represented by superoxide dismutase, catalase, glutathione reductase activity), interleukin-1 also measured. Results Restraint induced severe oxidative hepatic injury, as evidenced marked elevation ALT AST levels, increased species peroxidation activity, depletion enzyme activities. pretreatment significantly attenuated not only but increase peroxidation. It restored significant fraction reductase, peroxidase tissues. partially normalized interleukin-1β gene protein expression. Conclusions These results suggest that potent protective against via activities, have clinical applications field herbal medicine.